Discriminating glacial races of burbot (Lota lota) by means of PCR‐RF‐SSCP (PRS) analysis of the mitochondrial control region
Identifieur interne : 000867 ( Main/Exploration ); précédent : 000866; suivant : 000868Discriminating glacial races of burbot (Lota lota) by means of PCR‐RF‐SSCP (PRS) analysis of the mitochondrial control region
Auteurs : J. K. J. Van Houdt [Belgique] ; L. De Cleyn [Belgique] ; A. Perretti [Italie] ; F. A. M. Volckaert [Belgique]Source :
- Molecular Ecology Notes [ 1471-8278 ] ; 2006-06.
English descriptors
- KwdEn :
Abstract
We used closely related haplotypes of the mitochondrial control region of burbot (Lota lota) (Teleostei; Gadidae) with known sequences to assess the sensitivity of a standardized PRS (PCR‐RF‐SSCP) approach. In total, 537 pairwise comparisons between PRS patterns were evaluated. Only seven mutations did not result in a detectable change that resulted in 11 pairwise comparisons (2%) with indistinguishable haplotypes. The haplotypes that could not be discriminated were always characteristic for a certain assemblage (glacial race). As such, this method provides a reliable alternative to direct sequencing for phylogeographic studies in case intrapopulation diversity is limited.
Url:
DOI: 10.1111/j.1471-8286.2006.01268.x
Affiliations:
- Belgique, Italie
- Province du Brabant flamand, Région flamande
- Louvain
- Katholieke Universiteit Leuven
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